Goat anti-CB1 (aa44-58) Antibody

Pep ELISA, WB

Goat anti-CB1 (aa44-58) Antibody
Principle Name: CB1 (aa44-58)
Official Symbol: CNR1
All Name and Symbols: CNR1; cannabinoid receptor 1 (brain); CANN6; CB-R; CB1; CB1A; CB1K5; CB1R; CNR; OTTHUMP00000016839; cannabinoid receptor 1; central cannabinoid receptor
Accession Number(s): NP_057167.2; NP_149421.2
Peptide Sequence: NITEFYNKSLSSFKE, is from internal region (near N Terminus)
Human Gene ID(s): 1268
Non-Human GeneID(s): 12801 (mouse); 25248 (rat);
Important Comments: This antibody is expected to recognize both reported isoforms (NP_057167.2; NP_149421.2). Reported variants represent identical protein: NP_001153731.1, NP_001153732.1, NP_057167.2, NP_001153730.1, NP_001153698.1
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx 70+55kDa bands observed in Rat Brain lysates (calculated MW of 52.9kDa according to Human NP_057167.2 and 52.8kDa according to Rat NP_036916.1).The observed molecular weight corresponds to earlier findings in literature with differen
Reference(s): Scrima M, Di Marino S, Grimaldi M, Mastrogiacomo A, Novellino E, Bifulco M,
D'Ursi AM. Binding of the hemopressin peptide to the cannabinoid CB1 receptor:
structural insights. Biochemistry. 2010 Dec 14; 49(49):10449-57.PMID: 21062041