Goat anti-Neurofascin (aa877-890) Antibody

Pep ELISA, WB

Goat anti-Neurofascin (aa877-890) Antibody
Principle Name: Neurofascin (aa877-890)
Official Symbol: NFASC
All Name and Symbols: NFASC; neurofascin; DKFZp686P2250; FLJ46866; KIAA0756; NF; NRCAML; OTTHUMP00000065316; OTTHUMP00000065319; OTTHUMP00000065322; OTTHUMP00000065323; OTTHUMP00000065324; neurofascin homolog
Accession Number(s): NP_001005388.2; NP_001153803.1; NP_001153804.1; NP_055905.2;
Peptide Sequence: NFSPNQTKFTVQRT, is from internal region
Human Gene ID(s): 23114
Non-Human GeneID(s): 269116 (mouse); 116690 (rat);
Important Comments: This antibody is expected to recognize reported isoforms 1, 2, 3 and 4 (NP_001005388.2; , NP_001153803.1, NP_001153804.1and NP_055905.2 resp) .
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.Peptide ELISA: antibody detection limit dilution 1 to 16000.
Western Blot: Approx 170kDa band observed in Human Lung and Rat Brain lysates (calculated MW of 138kDa according to NP_001005388.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Kirschbaum et al,
Reference(s): Liu H, Focia PJ, He X. Homophilic adhesion mechanism of neurofascin, a member
of the L1 family of neural cell adhesion molecules. J Biol Chem. 2011 Jan 7; 286(1):797-805.PMID: 21047790