Goat anti-ATXN1 (aa686-699) Antibody

Goat anti-ATXN1 (aa686-699) Antibody
Principle Name: ATXN1 (aa686-699)
Official Symbol: ATXN1
All Name and Symbols: ATXN1; ataxin 1; ATX1; D6S504E; SCA1; OTTHUMP00000016065; ataxin-1; spinocerebellar ataxia type 1 protein
Accession Number(s): NP_000323.2
Peptide Sequence: TLKNLKNGSVKKGQ, is from internal region
Human Gene ID(s): 6310
Non-Human GeneID(s): 20238 (mouse); 25049 (rat);
Important Comments: Reported variants represent identical protein: NP_001121636.1, NP_000323.2
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Preliminary experiments gave bands at approx 75kDa and 26kDa in Mouse Fetal Brain lysates after 0.5ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calcul
Reference(s): Duvick L, Barnes J, Ebner B, Agrawal S, Andresen M, Lim J, Giesler GJ, Zoghbi HY, Orr HT, SCA1-like disease in mice expressing wild-type ataxin-1 with a serine to aspartic acid replacement at residue 776. Neuron 2010 Sep 67 (6): 929-35.PMID: 20869591