Goat anti-Caspase 3 Antibody

Pep ELISA, WB

Goat anti-Caspase 3 Antibody
Principle Name: Caspase 3
Official Symbol: CASP3
All Name and Symbols: CASP3; caspase 3; caspase 3, apoptosis-related cysteine protease; HGNC:1504; caspase 3, apoptosis-related cysteine peptidase; CPP32; CPP32B; SCA-1; PARP cleavage protease; SREBP cleavage activity 1; Yama; apopain; cysteine protease CPP32; procaspase3
Accession Number(s): NP_004337.2; NP_116786.1
Peptide Sequence: RDVSKEDHSKRS, is from internal region
Human Gene ID(s): 836
Important Comments: This antibody is expected to recognise both reported isoforms (NP_004337.2 and NP_116786.1).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx 30kDa band observed in lysates of cell line MOLT4 (calculated MW of 31.6kDa according to NP_004337.2; ). Recommended concentration: 1-3ug/ml. An additional band of unknown identity was also consistently observed at 65kDa. This band wa