Goat anti-GAD25 / GAD67 Antibody

Pep ELISA, WB

Goat anti-GAD25 / GAD67 Antibody
Principle Name: GAD25 / GAD67
Official Symbol: GAD1
All Name and Symbols: GAD1; glutamate decarboxylase 1 (brain, 67kDa); FLJ45882; GAD; SCP; 67 kDa glutamic acid decarboxylase; GAD-67; OTTHUMP00000204990; OTTHUMP00000204993; OTTHUMP00000204994; OTTHUMP00000204995; glutamate decarboxylase 1; glutamate decarboxylase 67 kDa isofo
Accession Number(s): NP_038473.2; NP_000808.2
Peptide Sequence: QRTNSLEEKSR, is from internal region
Human Gene ID(s): 2571
Important Comments: This antibody is expected to recognize both reported isoforms (NP_038473.2; NP_000808.2).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 8000.
Western Blot: Approx 70kDa band observed in Mouse Brain lysates (calculated MW of 66.6kDa according to Mouse NP_032103.2). Recommended concentration: 1-3ug/ml.
Reference(s): Wang G, Wang R, Ferris B, Salit J, Strulovici-Barel Y, Hackett NR, Crystal RG. Smoking-mediated up-regulation of GAD67 expression in the human airway
epithelium Respir Res. 2010 Oct 29; 11:150.PMID: 21034448