Goat anti-Ki-67 / MKI67 Antibody

Goat anti-Ki-67 / MKI67 Antibody
Principle Name: Ki-67 / MKI67
Official Symbol: MKI67
All Name and Symbols: MKI67; antigen identified by monoclonal antibody Ki-67 ; HGNC:7107; KIA; Ki-67; Proliferation-related Ki-67 antigen; monoclonal antibody Ki-67
Accession Number(s): NP_002408.3
Peptide Sequence: CVKKITTRSHRDSED, is from C Terminus
Human Gene ID(s): 4288
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as large as the calculated size of 359kDa according to NP_002408.3. Therefore we cannot recommend an optimal concentration.
Reference(s): Martin B, Paesmans M, Mascaux C, Berghmans T, Lothaire P, Meert AP, Lafitte JJ, Sculier JP. Ki-67 expression and patients survival in lung cancer: systematic review of the literature with meta-analysis. Br J Cancer. 2004 Dec 13; 91(12):2018-25. .PMID: 15545971