Goat anti-ZIC3 (aa205-218) Antibody

Goat anti-ZIC3 (aa205-218) Antibody
Principle Name: ZIC3 (aa205-218)
Official Symbol: ZIC3
All Name and Symbols: ZIC3; Zic family member 3 (odd-paired homolog, Drosophila); HTX; HTX1; ZNF203; heterotaxy 1; zinc finger protein ZIC 3; zinc finger protein of the cerebellum 3
Accession Number(s): NP_003404.1
Peptide Sequence: PRTDPYAAGAQFPN, is from internal region
Human Gene ID(s): 7547
Non-Human GeneID(s): 22773 (mouse); 367944 (rat);
Important Comments: This antibody is expected to NOT cross-react with other ZIC proteins.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA
Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Preliminary experiments in Human Brain lysates gave no specific signal but low background (at antibody concentration up to 1ug/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibo
Reference(s): Hatayama M, Tomizawa T, Sakai-Kato K, Bouvagnet P, Kose S, Imamoto N, Yokoyama S, Utsunomiya-Tate N, Mikoshiba K, Kigawa T, Aruga J, Functional and structural basis of the nuclear localization signal in the ZIC3 zinc finger domain. Human molecular genetics 2008 Nov 17 (22): 3459-73.PMID: 18716025