Goat anti-ARF4 (aa137-150) Antibody

Pep ELISA, WB, IHC

Goat anti-ARF4 (aa137-150) Antibody
Principle Name: ARF4 (aa137-150)
Official Symbol: ARF4
All Name and Symbols: ARF4; ADP-ribosylation factor 4; ARF2; ADP-ribosylation factor 2; OTTHUMP00000213140; OTTHUMP00000213142; OTTHUMP00000213211
Accession Number(s): NP_001651.1
Peptide Sequence: SEMTDKLGLQSLRN, is from internal region
Human Gene ID(s): 378
Non-Human GeneID(s): 11843 (mouse); 79120 (rat);
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Approx 19kDa band observed in lysates of cell lines NIH3T3 and HeLa (calculated MW of 20.5kDa according to NP_001651.1). Recommended concentration: 0.3-1ug/ml.
Immunohistochemistry: Paraffin embedded Human Thymus. Recommended concentration: 5ug/ml.
Reference(s): Jang SY, Jang SW, Ko J. Regulation of ADP-ribosylation factor 4 expression by small leucine zipper protein and involvement in breast cancer cell migration. Cancer Lett. 2012 Jan 28; 314(2):185-97.PMID: 22004728