Goat anti-Synaptotagmin-9 Antibody

Pep ELISA, WB

Goat anti-Synaptotagmin-9 Antibody
Principle Name: Synaptotagmin-9
Official Symbol: SYT9
All Name and Symbols: SYT9; synaptotagmin IX; synaptotagmin 9; sytIX
Accession Number(s): NP_783860.1
Peptide Sequence: EHDSCQDFIYHLRD, is from N Terminus
Human Gene ID(s): 143425
Non-Human GeneID(s): 60510 (mouse); 60564 (rat);
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 64000.
Western Blot: Approx 50kDa band observed in Mouse Liver, Pancreas and Thymus lysates (calculated MW of 56.3kDa according to NP_068689.2). Recommended concentration: 0.5-2ug/ml.
Reference(s): Boal F, Laguerre M, Milochau A, Lang J, Scotti PA. A charged prominence in the linker domain of the cysteine-string protein Cspa mediates its regulated interaction with the calcium sensor synaptotagmin 9 during exocytosis. FASEB J. 2011 Jan; 25(1):132-43.PMID: 20847230