Goat anti-Endophilin-A1 / SH3GL2 Antibody

Pep ELISA, WB, IHC

Goat anti-Endophilin-A1 / SH3GL2 Antibody
Principle Name: Endophilin-A1 / SH3GL2
Official Symbol: SH3GL2
All Name and Symbols: SH3GL2; SH3-domain GRB2-like 2; CNSA2; EEN-B1; FLJ20276; FLJ25015; SH3D2A; SH3P4; Endophilin A1 BAR domain; OTTHUMP00000021084; SH3 domain protein 2A; SH3 domain-containing GRB2-like protein 2; bA335L15.1 (SH3-domain GRB2-like 2); endophilin-1; endophilin
Accession Number(s): NP_003017.1
Peptide Sequence: SLEFPTGDSTQPN, is from internal region
Human Gene ID(s): 6456
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 64000.
Western Blot: Approx 38kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 40.0kDa according to NP_003017.1). Recommended concentration: 0.3-1ug/ml.
Immunohistochemistry: In paraffin embedded Human Cerebral Cortex shows staining of cytoplasm in some but not all neuronal cells. Recommended concentration, 5-10ug/ml.
Reference(s): Shang C, Guo Y, Fu S, Fu W, Sun K. SH3GL2 gene participates in MEK-ERK signal pathway partly by regulating EGFR in the laryngeal carcinoma cell line Hep2. Med Sci Monit. 2010 Jun; 16(6):BR168-73.PMID: 20512084