Goat anti-Alpha-synuclein Antibody

Pep ELISA, WB

Goat anti-Alpha-synuclein Antibody
Principle Name: Alpha-synuclein
Official Symbol: SNCA
All Name and Symbols: SNCA; synuclein, alpha (non A4 component of amyloid precursor); NACP; PARK1; PARK4; PD1; alpha-synuclein; non A-beta component of AD amyloid; synuclein alpha-140
Accession Number(s): NP_009292.1; NP_000336.1
Peptide Sequence: ATGFVKKDQLGK, is from internal region
Human Gene ID(s): 6622
Non-Human GeneID(s): 20617 (mouse); 29219 (rat);
Important Comments: This antibody is expected to recognize both reported isoforms (NP_009292.1; NP_000336.1). Reported variants represent identical protein: NP_000336.1, NP_001139527.1, NP_001139526.1
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Approx 17kDa band observed in Human Brain (Amygdala) and fetal Mouse Brain lysates (calculated MW of 14.5kDa according to Human NP_000336.1 and Mouse NP_033247.1). The observed molecular weight corresponds to earlier findings with different
Reference(s): Oueslati A, Paleologou KE, Schneider BL, Aebischer P, Lashuel HA. Mimicking phosphorylation at serine 87 inhibits the aggregation of human a-synuclein and protects against its toxicity in a rat model of Parkinson's disease. J Neurosci. 2012 Feb 1; 32(5):1536-44.PMID: 22302797