Goat anti-cytokeratin 20 (aa48-60) Antibody

Goat anti-cytokeratin 20 (aa48-60) Antibody
Principle Name: cytokeratin 20 (aa48-60)
Official Symbol: KRT20
All Name and Symbols: KRT20; keratin 20; CK-20; CK20; K20; KRT21; MGC35423; OTTHUMP00000164518; cytokeratin 20; cytokeratin-20; keratin, type I cytoskeletal 20; keratin-20; protein IT
Accession Number(s): NP_061883.1
Peptide Sequence: NSRHTVNYGSDLT, is from internal region
Human Gene ID(s): 54474
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB, EIA
Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Approx 49kDa band observed in Human Colon and Ileum lysates (calculated MW of 48.5kDa according to NP_061883.1). Recommended concentration: 0.01-0.03ug/ml.
Reference(s): Magnusson K, de Wit M, Brennan DJ, Johnson LB, McGee SF, Lundberg E, Naicker K, Klinger R, Kampf C, Asplund A, Wester K, Gry M, Bjartell A, Gallagher WM, Rexhepaj E, Kilpinen S, Kallioniemi OP, Belt E, Goos J, Meijer G, Birgisson H, Glimelius B, Borrebaec SATB2 in combination with cytokeratin 20 identifies over 95% of all colorectal carcinomas. Am J Surg Pathol. 2011 Jul; 35(7):937-48.PMID: 21677534