Goat anti-C5AR1/CD88 Antibody

Goat anti-C5AR1/CD88 Antibody
Principle Name: C5AR1/CD88
Official Symbol: C5AR1
All Name and Symbols: C5AR1; C5A, C5AR; C5A-R; C5R1; CD88; C5a anaphylatoxin chemotactic receptor; C5a anaphylatoxin receptor; Complement component 5 receptor 1; Complement component 5a receptor 1; Anaphylatoxin receptor
Accession Number(s): NP_001727.1
Peptide Sequence: RESKSFTRSTVDT, is from C Terminus
Human Gene ID(s): 728
Non-Human GeneID(s): 113959 (rat);
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB, IF, FC
Peptide ELISA: antibody detection limit dilution 1 to 64000.
Western Blot: Approx 38kDa band observed in lysates of cell line HepG2 (calculated MW of 39.3kDa according to NP_001727.1). Recommended concentration: 0.01-0.03ug/ml. Primary incubation 1 hour at room temperature.
Reference(s): Hosgood HD, Zhang L, Shen M, Berndt SI, Vermeulen R, Li G, Yin S, Yeager M, Yuenger J, Rothman N, Chanock S, Smith M, Lan Q, Association between genetic variants in VEGF, ERCC3 and occupational benzene haematotoxicity. Occupational and environmental medicine 2009 Dec 66 (12): 848-53.PMID: 19773279