Goat anti-EEF1A2 Antibody

Pep ELISA, WB

Goat anti-EEF1A2 Antibody
Principle Name: EEF1A2
Official Symbol: EEF1A2
All Name and Symbols: EEF1A2; eukaryotic translation elongation factor 1 alpha 2; EEF1AL; EF-1-alpha-2; EF1A; FLJ41696; HS1; STN; STNL; OTTHUMP00000031776; eEF1A-2; elongation factor 1-alpha 2; elongation factor-1 alpha; eukaryotic elongation factor 1 A-2; statin S1; statin-S1
Accession Number(s): NP_001949.1
Peptide Sequence: NPATVPFVPIS, is from internal region
Human Gene ID(s): 1917
Non-Human GeneID(s): 13628 (mouse); 24799 (rat);
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.Peptide ELISA: antibody detection limit dilution 1 to 8000.
Western Blot: Approx 50kDa band observed in lysates of cell lines Daudi and Jurkat (calculated MW of 50.5kDa according to NP_001949.1). The same band is observed in lysates of Mouse and Rat Heart and Skeletal Muscle. Recommended concentration: 0.5-2ug/ml
Reference(s): Li Z, Qi CF, Shin DM, Zingone A, Newbery HJ, Kovalchuk AL, Abbott CM, Morse HC 3rd. Eef1a2 promotes cell growth, inhibits apoptosis and activates JAK/STAT and AKT signaling in mouse plasmacytomas. PLoS One. 2010 May 21; 5(5):e10755.PMID: 20505761