Goat anti-polo-like kinase 3 (aa320-333) Antibody

Pep ELISA, WB, IHC

Goat anti-polo-like kinase 3 (aa320-333) Antibody
Principle Name: polo-like kinase 3 (aa320-333)
Official Symbol: PLK3
All Name and Symbols: PLK3; polo-like kinase 3; CNK; FNK; PRK; FGF-inducible kinase; PLK-3; cytokine-inducible kinase; cytokine-inducible serine/threonine-protein kinase; proliferation-related kinase; serine/threonine-protein kinase PLK3
Accession Number(s): NP_004064.2
Peptide Sequence: PDRLPISSCVTVPD, is from internal region
Human Gene ID(s): 1263
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Approx 70kDa band observed in lysates of cell line HeLa (calculated MW of 71.6kDa according to NP_004064.2). Recommended concentration: 1-3ug/ml.
Immunohistochemistry: In paraffin embedded Human Kidney shows vesicular staining in the cystoplasm of collective tubules. Recommended concentration, 5-10ug/ml.
Reference(s): Wang J, Beauchemin M, Bertrand R. Bcl-xL phosphorylation at Ser49 by polo kinase 3 during cell cycle progression and checkpoints. Cell Signal. 2011 Dec; 23(12):2030-8.PMID: 21840391