Goat anti-Myosin IH Antibody

Pep ELISA, WB, IHC

Goat anti-Myosin IH Antibody
Principle Name: Myosin IH
Official Symbol: Myo1H
All Name and Symbols: MYO1H; myosin IH; myosin-1H; myosin-Ih; unconventional myosin-Ih
Accession Number(s): NP_001158045.1
Peptide Sequence: EDQVYKDKNGQLR, is from C Terminus
Human Gene ID(s): 283446
Non-Human GeneID(s): 231646 (mouse); 687866 (rat);
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Approx 110kDa band observed in lysates of cell line NIH3T3 (calculated MW of 118kDa according to NP_001158045.1). Recommended concentration: 0.1-0.3ug/ml. An additional band of unknown identity was also consistently observed at 28kDa. This
Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 5ug/ml.
Reference(s): Tassopoulou-Fishell M, Deeley K, Harvey EM, Sciote J, Vieira AR. Genetic variation in myosin 1H contributes to mandibular prognathism. American journal of orthodontics and dentofacial orthopedics : official publication of the American Association of Orthodontists, its constituent societies, and the American Board of Orthodontics 2012 Jan 141 (1): 51-9.PMID: 22196185