Goat anti-APOD Antibody

Goat anti-APOD Antibody
Principle Name: APOD
Official Symbol: APOD
All Name and Symbols: APOD; apolipoprotein D; apo-D
Accession Number(s): NP_001638.1
Peptide Sequence: EIEKIPTTFENGRC, is from internal region
Human Gene ID(s): 347
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB, EIA, IHC
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx 25kDa band observed in Human Liver and Kidney lysates (calculated MW of 21.3kDa according to NP_001638.1). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.3-1ug/ml.
Immunohistochemistry: In paraffin embedded Human Kidney shows staining of the brush border of PCT. Recommended concentration, 5-10ug/ml.
Reference(s): Chen H, Li Y, Du J, Cao Y, Li X. Increased JNK1 activity contributes to the upregulation of ApoD in the apocrine secretory gland cells from axillary osmidrosis. Mol Cell Biochem. 2011 Aug; 354(1-2):311-6.PMID: 21526344