Goat anti-keratin 18 Antibody

Pep ELISA, WB

Goat anti-keratin 18 Antibody
Principle Name: keratin 18
Official Symbol: KRT18
All Name and Symbols: KRT18; keratin 18; CYK18; K18; CK-18; cell proliferation-inducing gene 46 protein; cell proliferation-inducing protein 46; cytokeratin 18; cytokeratin-18; keratin, type I cytoskeletal 18; keratin-18
Accession Number(s): NP_000215.1
Peptide Sequence: EELDKYWSQQIE, is from internal region
Human Gene ID(s): 3875
Non-Human GeneID(s): 16668 (mouse); 294853 (rat);
Important Comments: Reported variants represent identical protein: NP_000215.1, NP_954657.1.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx 48kDa band observed in lysates of cell lines A431, HeLa, MCF7 (calculated MW of 48.1kDa according to NP_000215.1) and approx 45kDa in lysates of Pig Liver (calculated MW of 47.4kDa according to XP_005652636.2). Recommended concentrat
Reference(s): Meng Y, Wu Z, Yin X, Zhao Y, Chen M, Si Y, Yang J, Fu X, Han W. Keratin 18 attenuates estrogen receptor alpha-mediated signaling by sequestering LRP16 in cytoplasm. BMC cell biology 2009 10 : 96.PMID: 20035625