Goat anti-BMI1 (aa237-251) Antibody

Pep ELISA, WB

Goat anti-BMI1 (aa237-251) Antibody
Principle Name: BMI1 (aa237-251)
Official Symbol: BMI1
All Name and Symbols: BMI1; BMI1 polycomb ring finger oncogene; FLVI2/BMI1; PCGF4; RNF51; B lymphoma Mo-MLV insertion region 1 homolog; flvi-2/bmi-1; murine leukemia viral (bmi-1) oncogene homolog; polycomb complex protein BMI-1; polycomb group RING finger protein 4; polycomb
Accession Number(s): NP_005171.4
Peptide Sequence: SHQRDGLTNAGELES, is from internal region
Human Gene ID(s): 648
Non-Human GeneID(s): 12151 (mouse);
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Approx 40kDa band observed in nuclear lysates of cell lines K562 and NIH3T3 (calculated MW of 36.9kDa according to NP_005171.4). This band corresponds to earlier findings in literature with different antibodies (Chagraoui et al, Genes Dev.
Reference(s): Cho JH, Dimri M, Dimri GP. A positive feedback loop regulates the expression of polycomb group protein BMI1 via WNT signaling pathway. The Journal of biological chemistry 2013 Feb 288 (5): 3406-18.PMID: 23239878