Goat anti-CCAR2 / DBC1 (aa842-855) Antibody

Pep ELISA, WB

Goat anti-CCAR2 / DBC1 (aa842-855) Antibody
Principle Name: CCAR2 / DBC1 (aa842-855)
Official Symbol: CCAR2
All Name and Symbols: CCAR2; cell cycle and apoptosis regulator 2; DBC-1; DBC1; KIAA1967; NET35; p30 DBC; p30DBC; cell cycle and apoptosis regulator protein 2; cell division cycle and apoptosis regulator protein 2; deleted in breast cancer 1; p30 DBC protein
Accession Number(s): NP_066997.3
Peptide Sequence: ESHNRFSATEVTNK, is from internal region
Human Gene ID(s): 57805
Non-Human GeneID(s): 219158 (mouse); 306007 (rat);
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 64000.
Western Blot: Approx 140kDa band observed in nuclear lysates of cell lines HEK293, MCF7 and NIH3T3 (calculated MW of 103kDa according to Human NP_066997.3 and Mouse NP_666167.3). The observed molecular weight is explained by its acetylation (Zheng et al
Reference(s): Kim SH, Kim JH, Yu EJ, Lee KW, Park CK. The overexpression of DBC1 in esophageal squamous cell carcinoma correlates with poor prognosis. Histology and histopathology 2012 Jan 27 (1): 49-58.PMID: 22127596