Goat anti-CTNNB1 / catenin beta-1 (aa108-121) Antibody

Goat anti-CTNNB1 / catenin beta-1 (aa108-121) Antibody
Principle Name: CTNNB1 / catenin beta-1 (aa108-121)
Official Symbol: CTNNB1
All Name and Symbols: CTNNB1; catenin (cadherin-associated protein), beta 1, 88kDa; CTNNB; MRD19; armadillo; catenin beta-1
Accession Number(s): NP_001895.1
Peptide Sequence: QIPSTQFDAAHPTN, is from internal region
Human Gene ID(s): 1499
Non-Human GeneID(s): 12387 (mouse); 84353 (rat);
Important Comments: Reported variants represent identical protein: NP_001091679.1, NP_001895.1, NP_001091680.1.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, IF, WB
Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Approx 100kDa band observed in lysates of cell line HeLa (calculated MW of 85.5kDa according to NP_001895.1). Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature.
Reference(s): Mazemondet O, John M, Leye S, Rolfs A, Uhrmacher AM. Elucidating the sources of ß-catenin dynamics in human neural progenitor cells. PloS one 2012 7 (8): e42792.PMID: 22952611