Goat anti-GAPDH (C Terminus) Loading Control, biotinylated Antibody

Goat anti-GAPDH (C Terminus) Loading Control, biotinylated Antibody
Principle Name: GAPDH (C Terminus) Loading Control, biotinylated
Official Symbol: GAPDH
All Name and Symbols: GAPDH; glyceraldehyde-3-phosphate dehydrogenase; G3PD; GAPD; HEL-S-162eP; aging-associated gene 9 protein; epididymis secretory sperm binding protein Li 162eP; peptidyl-cysteine S-nitrosylase GAPDH
Accession Number(s): NP_002037.2
Peptide Sequence: HQVVSSDFNSDT., is from C Terminus
Human Gene ID(s): 2597
Important Comments: GAPDH is constitutively expressed in almost all tissues at high levels. It is therefore a useful marker when a loading/positive control is required in western blotting.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WBPeptide ELISA: antibody detection limit dilution 1 to 64000.
Western Blot: Approx 35kDa band observed in lysates of cel line HEK293 (calculated MW of 36.1kDa according to NP_002037.2). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.3-1ug/ml.
Reference(s): Burke JR, Enghild JJ, Martin ME, Jou YS, Myers RM, Roses AD, Vance JM, Strittmatter WJ. Huntingtin and DRPLA proteins selectively interact with the enzyme GAPDH. Nature medicine 1996 Mar 2 (3): 347-50.PMID: 8612237