Goat anti-GAPDH (Internal), Biotinylated Antibody

Pep ELISA, WB, IHC

Goat anti-GAPDH (Internal), Biotinylated Antibody
Principle Name: GAPDH (Internal), Biotinylated
Official Symbol: GAPDH
All Name and Symbols: GAPDH; glyceraldehyde-3-phosphate dehydrogenase; G3PD; GAPD; HEL-S-162eP; aging-associated gene 9 protein; epididymis secretory sperm binding protein Li 162eP; peptidyl-cysteine S-nitrosylase GAPDH
Accession Number(s): NP_002037.2; NP_001243728.1
Peptide Sequence: GVNHEKYDNSLK., is from internal region
Human Gene ID(s): 2597
Non-Human GeneID(s): 14433 (mouse); 24383 (rat);
Important Comments: This antibody is expected to recognize both reported isoforms (NP_002037.2; NP_001243728.1). Reported variants represent identical protein: NP_001276674.1, NP_002037.2, NP_001276675.1. GAPDH is constitutively expressed in almost all tissues at high levels
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.Peptide ELISA: antibody detection limit dilution 1 to 16000.
Western Blot: Approx 36kDa band observed in Human Liver lysates (calculated MW of 36.1kDa according to NP_002037.2). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 1-3ug/ml.
Reference(s): Ismail SA, Park HW. Structural analysis of human liver glyceraldehyde-3-phosphate dehydrogenase. Acta crystallographica. Section D, Biological crystallography 2005 Nov 61 (Pt 11): 1508-13.PMID: 16239728