Goat anti-Argininosuccinate synthetase 1, Biotinylated Antibody

Goat anti-Argininosuccinate synthetase 1, Biotinylated Antibody
Principle Name: Argininosuccinate synthetase 1, Biotinylated
Official Symbol: ASS1
All Name and Symbols: ASS1; argininosuccinate synthase 1; ASS; CTLN1; argininosuccinate synthetase 1; citrulline--aspartate ligase; citrulline-aspartate ligase
Accession Number(s): NP_000041.2
Peptide Sequence: ENPKNQAPPGLYTKTQD., is from internal region
Human Gene ID(s): 445
Non-Human GeneID(s): 11898 (mouse); 25698 (rat);
Important Comments: Reported variants represent identical protein: NP_446464.1, NP_000041.2
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB, IF, IP, IHCPeptide ELISA: antibody detection limit dilution 1 to 64000.
Western Blot: Approx 46kDa band observed in Human Kidney lysates (calculated MW of 46.5kDa according to NP_000041.2). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.01-0.03ug/ml.
Reference(s): Hao G, Xie L, Gross SS. Argininosuccinate synthetase is reversibly inactivated by S-nitrosylation in vitro and in vivo. The Journal of biological chemistry 2004 Aug 279 (35): 36192-200.PMID: 15192091