Goat anti-Ketohexokinase Antibody

Pep ELISA, WB

Goat anti-Ketohexokinase Antibody
Principle Name: Ketohexokinase
Official Symbol: KHK
All Name and Symbols: KHK; ketohexokinase (fructokinase); hepatic fructokinase; ketohexokinase
Accession Number(s): NP_000212.1; NP_006479.1
Peptide Sequence: RCLSQRWQRGGNASN , is from internal region
Human Gene ID(s): 3795
Non-Human GeneID(s): 16548 (mouse); 25659 (rat);
Important Comments: This antibody is expected to recognize both reported isoforms (NP_000212.1; NP_006479.1).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Approx 30kDa band observed in Human, Mouse, Rat and Pig Liver lysates (calculated MW of 32.7kDa according to Human NP_000212.1, Mouse NP_032465.2, Rat NP_114061.1 and of 32.6kDa according to Pig XP_003125348.1). Recommended concentration: 0
Reference(s): Asipu A, Hayward BE, O'Reilly J, Bonthron DT. Properties of normal and mutant recombinant human ketohexokinases and implications for the pathogenesis of essential fructosuria. Diabetes 2003 Sep 52 (9): 2426-32.PMID: 12941785