Goat anti-Insulysin / Insulinase, Biotinylated Antibody

Pep ELISA, WB, IHC

Goat anti-Insulysin / Insulinase, Biotinylated Antibody
Principle Name: Insulysin / Insulinase, Biotinylated
Official Symbol: IDE
All Name and Symbols: IDE; insulin degrading enzyme; INSULYSIN; Abeta-degrading protease; insulin protease; insulinase
Accession Number(s): NP_004960.2; NP_001159418.1; NP_001309722.1; NP_001309723.1; NP_001309724.1; NP_001309726.1
Peptide Sequence: CQQYNFDRDNTE., is from internal region
Human Gene ID(s): 3416
Important Comments: This antibody is expected to recognize all reported isoforms (NP_004960.2; NP_001159418.1; NP_001309722.1; NP_001309723.1; NP_001309724.1; NP_001309726.1). Reported variants represent identical protein: NP_001309725.1, NP_001309724.1
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx 120kDa band observed in lysates of cell line K562 (calculated MW of 118kDa according to NP_004960.2). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.3-1ug/ml.
Reference(s): Zhao Z, Xiang Z, Haroutunian V, Buxbaum JD, Stetka B, Pasinetti GM. Insulin degrading enzyme activity selectively decreases in the hippocampal formation of cases at high risk to develop Alzheimer's disease. Neurobiology of aging 2007 Jun 28 (6): 824-30.PMID: 16769157