Goat anti-Flotillin 1, Biotinylated Antibody

Pep ELISA, WB, IHC

Goat anti-Flotillin 1, Biotinylated Antibody
Principle Name: Flotillin 1, Biotinylated
Official Symbol: FLOT1
All Name and Symbols: FLOT1; flotillin 1; integral membrane component of caveolae
Accession Number(s): NP_005794.1; NP_001305804.1
Peptide Sequence: CSISQVNHKPLRTA., is from C Terminus
Human Gene ID(s): 10211
Important Comments: This antibody is expected to recognize both reported isoforms (NP_005794.1; NP_001305804.1).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 2000.
Western Blot: Approx 48kDa band observed in lysates of cell line K562 (calculated MW of 47.4kDa according to NP_005794.1). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 1-3ug/ml.
Reference(s): Bickel PE, Scherer PE, Schnitzer JE, Oh P, Lisanti MP, Lodish HF. Flotillin and epidermal surface antigen define a new family of caveolae-associated integral membrane proteins. The Journal of biological chemistry 1997 May 272 (21): 13793-802.PMID: 9153235