Goat anti-B7-H3 / CD276 Antibody

Goat anti-B7-H3 / CD276 Antibody
Principle Name: B7-H3 / CD276
Official Symbol: CD276
All Name and Symbols: CD276; CD276 molecule; 4Ig-B7-H3; B7-H3; B7H3; B7RP-2; B7 homolog 3; costimulatory molecule
Accession Number(s): NP_001019907.1; NP_079516.1
Peptide Sequence: KHSDSKEDDGQE, is from C Terminus
Human Gene ID(s): 80381
Non-Human GeneID(s):
Important Comments:
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 8000.
Western Blot: Approx 55kDa band observed in Human Placenta and Testis lysates (calculated MW of 57.2kDa according to NP_001019907.1). Recommended concentration: 0.1-3ug/ml.
Reference(s): Chen JT, Chen CH, Ku KL, Hsiao M, Chiang CP, Hsu TL, Chen MH, Wong CH. Glycoprotein B7-H3 overexpression and aberrant glycosylation in oral cancer and immune response. Proceedings of the National Academy of Sciences of the United States of America 2015 Oct 112 (42): 13057-62..PMID: 26438868