Sheep Polyclonal anti-human TNF RI (TNFRSF1A)

Sheep Polyclonal anti-human TNF RI (TNFRSF1A)
Immunogen: E. coli-derived recombinant human TNF RI/TNFRSF1A
Preparation: This antibody was produced from a sheep immunized with purified human TNF RI/TNFRSF1A protein
Formulation and Storage: The IgG fraction of immunized serum was purified by antigen affinity chromatography and lyophilized from a 0.2 um filtered solution in phosphate-buffered saline (PBS). Lyophilized samples are stable for 2 years from the date of receipt when stored at -70C
Specificity: Detects human sTNF RI/TNFRSF1A in direct ELISAs and Western blots. In direct ELISAs and Western blots (non-reducing conditions), less than 10% cross-reactivity with recombinant mouse TNF sRI is observed. Additionally, in direct ELISAs, this antibody does not cross-react with recombinant human (rh) sTNF RII, recombinant mouse (rm) sTNF RII, rhTNF-alpha, rmTNF-alpha, rhTNF-beta. This anti-human TNF RI antibody can interact with the cell surface 55 kDa human TNF RI and exhibits TNF agonist activities on the A549 human lung carcinoma cell line. This antibody also exhibits TNF agonist activities on the L-929 mouse fibroblast cell line, suggesting that it has cross-species activity with mouse cell surface TNF RI.
Reconstitution: Reconstitute the antibody with 100 l sterile PBS and the final concentration is 500g/ml. Reconstituted antibody can also be aliquoted and stored frozen at < -20 C for at least for six months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Applications:
A.:WB-(1 to 1000-2000)
B.:ICC-(1 to 100-200)
C.:FC-Yes
D.: