Mouse Monoclonal Antibody to TNF-alpha

Mouse IgG1

Enterez GeneID: 7124
General Information: TNF-alpha (tumor necrosis factor alpha) is an important cytokine produced by numerous cell types including neutrophils, activated lymphocytes, macrophages and NK cells. It plays a critical role in inflammatory responses and in apoptosis. TNF-alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF-alpha exists as a multimer of two, three, or five non covalently linked units, but shows a single 17 kDa band following SDS PAGE under non reducing conditions. Although it has little effect on many cultured normal human cells, TNF-alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF-alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF-alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.
Immunogen: Recombinant Human TNF-alpha (BioSource company, Cat.No. PHC3013)
Size and Concentration: 100ug/1mg/ml
Supplied As: Lyophilized Powder from 100ul of Purified antibody in PBS containing 0.03% sodium azide.
Reconstitution and Storage: Reconstituted with 100ul sterile DI H2O, at stored at 4C or -20C for short or long term storage.
Applications: ELISA: 1 to 10000; WB: 1 to 500 - 1 to 2000
References: 1. Dean GA. LaVoy A. Burkhard MJ. Vet Immunol Immunopathol. 2004, Jul, 100(1-2):49-59. ; 2. Arens R. Schepers K. Nolte MA. et al. J Exp Med. 2004, Jun 7, 199(11):1595-605. ; 3. Podhorecka M. Dmoszynska A. Rolinski J. Eur J Haematol. 2004, Jul, 73(1):29-35. ;