Mouse Monoclonal Antibody to Vimentin

Mouse IgG1

MW: 54kDa
Enterez GeneID: 7431
General Information: Vimentin, also know as VIM. It is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors. They are generally used with a panel of other antibodies including those recognising cytokeratins, lymphoid markers, S100, desmin and neurofilaments.
Immunogen: Purified recombinant fragment of Vimentin (aa2-466) expressed in E. Coli. ;
Size and Concentration: 100ug/1mg/ml
Supplied As: Lyophilized Powder from 100ul of Ascitic fluid containing 0.03% sodium azide. ;
Reconstitution and Storage: Reconstituted with 100ul sterile DI H2O, at stored at 4C or -20C for short or long term storage.
Applications: ELISA: 1 to 10000; WB: 1 to 500 - 1 to 2000; IHC: 1 to 200 - 1 to 1000
References: 1. J Pediatr Hematol Oncol. 2007 May; 29(5):293-7. ; 2. PLoS ONE. 2008 Jun 30; 3(7):e2816.