Mouse Monoclonal Antibody to AFP

Mouse IgG2b

MW: 69kDa
Enterez GeneID: 174
General Information: AFP (alpha-fetoprotein), a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.
Immunogen: Purified recombinant fragment of human AFP expressed in E. Coli.
Size and Concentration: 100ug/1mg/ml
Supplied As: Lyophilized Powder from 100ul of Antibody are purified by protein G affinity chromatography. ; Liquid in PBS containing 50% glycerol and 0.03% sodium azide. ;
Reconstitution and Storage: Reconstituted with 100ul sterile DI H2O, at stored at 4C or -20C for short or long term storage.
Applications: ELISA: 1 to 10000; WB: 1 to 500 - 1 to 2000
References: 1. J Biol Chem. 1995 Oct 20; 270(42):25028-36. ; 2. Proc Natl Acad Sci U S A. 1994 Jul 5; 91(14):6374-8. ; 3. Leuk Lymphoma. 2002 Dec; 43(12):2355-62. ;