Mouse Monoclonal Antibody to Metadherin

Mouse IgG1

MW: 64kDa
Enterez GeneID: 92140
General Information: Metadherin (Metastasis adhesion protein), also known as MTDH, LYsine-RIch CEACAM1 co-isolated (LYRIC), is a novel protein that localizes with the tight junction proteins ZO-1 and occludin in polarized epithelial cells. At the tight junction, it acts not as a structural component, but is rather recruited during the maturation of the tight junction complex. Metadherin is overexpressed in breast cancer tissue and breast tumor xenografts, while much lower levels are expressed in normal breast tissue. Metadherin binds to lung vasculature, one of the four common sites of breast cancer metastasis, through a C-terminal segment in the extracellular domain; blocking this lung-homing domain with antibodies or inhibiting metadherin with siRNA has been reported to inhibit breast cancer metastasis.
Immunogen: Purified recombinant fragment of human Metadherin expressed in E. Coli.
Size and Concentration: 100ug/1mg/ml
Supplied As: Lyophilized Powder from 100ul of Antibody are purified by protein G affinity chromatography. ; Liquid in PBS containing 50% glycerol and 0.03% sodium azide. ;
Reconstitution and Storage: Reconstituted with 100ul sterile DI H2O, at stored at 4C or -20C for short or long term storage.
Applications: ELISA: 1 to 10000; WB: 1 to 500 - 1 to 2000; IHC: 1 to 200 - 1 to 1000; FCM: 1 to 200 - 1 to 400
References: 1. Nature. 1997 Aug 7; 388(6642):548-54. ; 2. Cell. 1998 Dec 11; 95(6):749-58. ; 3. J Biol Chem. 2000 Jun 16; 275(24):18180-7.