Serum Amyloid A (APO-SAA1) Human Recombinant

Lyophilized powder at room temperature.

SAA1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 104 amino acids and having a molecular mass of 11.7kDa. The SAA1 is purified by proprietary chromatographic techniques.
Amino Acid Sequence:RSFFSFLGEA FDGARDMWRA YSDMREANYI GSDKYFHARG NYDAAKRGPG GVWAAEAISD ARENIQRFFG HGAEDSLADQ AANEWGRSGK DPNHFRPAGL PEKY.
Physical Appearance and Stability: Lyophilized SAA1 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution SAA1 should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Formulation and Purity: SAA1 was lyophilized from a 0.2um filtered concentrated solution in 20mM Tris-HCl, pH 8.5 and 150mM NaCl. Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Solubility: It is recommended to reconstitute the lyophilized SAA1 in sterile 18MO-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Biological Activity: The biological activity determined by a chemoattract bioassay using human monocytes is in a concentration range of 10-100 ng/ml.