Tumor Necrosis Factor Receptor 2 Fusion Protein Human Recombinant

Lyophilized powder at room temperature.

Recombinant Human Tumor Necrosis Factor Receptor 2 Fusion Protein produced in CHO is a dimeric, glycosylated, polypeptide chain consisting of the extracellular ligand-binding portion of the human 75 kilo Dalton (p75) tumor necrosis factor receptor 2 (TNFR2) linked to the Fc portion of human IgG1. The Fc component of TNFR2 contains the CH2 domain, the CH3 domain and hinge region, but not the CH1 domain of IgG1. It consists of 934 amino acids and has an apparent molecular weight of approximately 150 kilo Daltons.The TNFR2 is purified by standard chromatographic techniques.
Source: Chinese Hamster Ovarian Cells (CHO).
Physical Appearance and Stability: Lyophilized Tumor Necrosis Factor Receptor although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution TNFR2 should be stored at 4C between 2-7 days and for future use below -18C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Formulation and Purity: Each mg contains 1.6mg mannitol, 0.4 mg sucrose and 48 ug tromethamine. Greater than 98.0% as determined by:(a) Analysis by RP-HPLC.(c) Analysis by SDS-PAGE.
Solubility: It is recommended to reconstitute the lyophilized TNFR2 in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Biological Activity: Potency is determined by its ability to neutralize TNF-alpha mediated growth inhibition of A375 cells, corresponding to a Specific Activity of 17, 000, 000 IU/mg.