Matrix Metalloproteinase-2 Human Recombinant, HEK

Lyophilized powder at room temperature.

MMP-2 Human Recombinant produced in HEK293 cells is a proform of the Human MMP-2 (Ala30-Cys660) and fused with a ployhistide tag at the C-terminus, having an Mw of 71kDa. MMP-2 is purified by proprietary chromatographic techniques.
Source: HEK293 cells.
Physical Appearance and Stability: Store MMP-2 at 4C if entire vial will be used within 2-4 weeks. Store frozen at -20C for longer periods of time.Avoid multiple freeze-thaw cycles.
Formulation and Purity: The MMP-2 is supplied as a 0.2um filtered solution in 20mM Tris-HCl, 150mM NaCl and 0.05% Brij 35, pH 7.4. Greater than 95% as determined by SDS-PAGE.
Biological Activity: The activity was measured by its ability to cleave fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2 (RND, Catalog # ES001)., The specific activity is > 1, 000 pmoles/min/ug.Recombinant Human MMP-2 protein pro form needs to be activated with p-aminophenylmercuric acetate (APMA).Activation Protocol:1. Dilute MMP2 to 100ug/ml in the Assay Buffer: 50mM Tris, 10mM CaCl2, 150mM NaCl, 0.05% (w/v) and Brij 35, pH 7.5.2. Activate MMP2 by adding APMA to a final concentration of 1mM. (Sigma, Catalog # A9563) and 100mM stock in DMSO.3. Incubate at 37C for 1 hour.