Calcium/Calmodulin-Dependent Protein Kinase IV Human Recombinant

Lyophilized powder at room temperature.

CAMK4 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 497 amino acids (1-473) and having a molecular mass of 54.5kDa.CAMK4 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSHMLKVTV PSCSASSCSS VTASAAPGTA SLVPDYWIDG SNRDALSDFF EVESELGRGA TSIVYRCKQK GTQKPYALKV LKKTVDKKIV RTEIGVLLRL SHPNIIKLKE IFETPTEISL VLELVTGGEL FDRIVEKGYY SERDAADAVK QILEAVAYLH ENGIVHRDLK PENLLYATPA PDAPLKIADF GLSKIVEHQV LMKTVCGTPG YCAPEILRGC AYGPEVDMWS VGIITYILLC GFEPFYDERG DQFMFRRILN CEYYFISPWW DEVSLNAKDL VRKLIVLDPK KRLTTFQALQ HPWVTGKAAN FVHMDTAQKK LQEFNARRKL KAAVKAVVAS SRLGSASSSH GSIQESHKAS RDPSPIQDGN EDMKAIPEGE KIQGDGAQAA VKGAQAELMK VQALEKVKGA DINAEEAPKM VPKAVEDGIK VADLELEEGL AEEKLKTVEE AAAPREGQGS SAVGFEVPQQ DVILPEY.
Physical Appearance and Stability: Store at 4C if entire vial will be used within 2-4 weeks. Store, frozen at -20C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Formulation and Purity: The CAMK4 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 100mM NaCl and 10% glycerol. Greater than 85% as determined by SDS-PAGE.