Glycogen synthase kinase-3 beta, sf9 Human Recombinant

Lyophilized powder at room temperature.

GSK3B produced in Sf9 Baculovirus cells is a single,
glycosylated polypeptide chain containing 426 amino acids (1-420a.a.) and
having a molecular mass of 47.5kDa (Migrates at 40-57kDa on SDS-PAGE under
reducing conditions).
GSK3B is expressed with a 6 amino acid His tag at
C-Terminus and purified by proprietary chromatographic techniques.
Amino Acid Sequence:MSGRPRTTSF
AESCKPVQQP SAFGSMKVSR DKDGSKVTTV VATPGQGPDR PQEVSYTDTK VIGNGSFGVV YQAKLCDSGE
LVAIKKVLQD KRFKNRELQI MRKLDHCNIV RLRYFFYSSG EKKDEVYLNL VLDYVPETVY RVARHYSRAK
QTLPVIYVKL YMYQLFRSLA YIHSFGICHR DIKPQNLLLD PDTAVLKLCD FGSAKQLVRG EPNVSYICSR
YYRAPELIFG ATDYTSSIDV WSAGCVLAEL LLGQPIFPGD SGVDQLVEII KVLGTPTREQ IREMNPNYTE
FKFPQIKAHP WTKVFRPRTP PEAIALCSRL LEYTPTARLT PLEACAHSFF DELRDPNVKL PNGRDTPALF NFTTQELSSN
PPLATILIPP HARIQAAAST PTNATAASDA NTGDRGQTNN AASASASNST HHHHHH.
Physical Appearance and Stability: Store at 4C if entire vial will be used within 2-4 weeks. Store, frozen at -20C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Formulation and Purity: GSK3B protein solution (0.25mg/ml) contains Phosphate
Buffered Saline (pH7.4), 0.5mM PMSF and 30% glycerol. Greater than 90.0% as determined by SDS-PAGE.