Tissue Inhibitor of Metalloprotease 2 Human Recombinant, HEK

Lyophilized powder at room temperature.

TIMP2 Human Recombinant produced in HEK-293 cells is a secreted protein with the sequence of Human TIMP-2 (amino acids Cys27-Pro220) and is fused to a polyhistidine tag at the C-terminus.
Source: HEK293 Cells.
Physical Appearance and Stability: Lyophilized TIMP2 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution TIMP2 should be stored at 4C between 2-7 days and for future use below -18C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Formulation and Purity: The TIMP2 protein was lyophilized after extensive dialysis against PBS. Greater than 95.0% as determined by SDS-PAGE.
Solubility: It is recommended to reconstitute the lyophilized TIMP2 in sterile assay buffer (50mM Tris, 10mM CaCl2, 150mM NaCl, 0.05% Brij-35, PH 7.5) not less than 100ug/ml.
Biological Activity: The IC50 of 3 nM is measured by its ability to inhibit recombinant human MMP-2 cleavage of the colorimetric peptide substrate, Mca-PLGL-DpaAR-NH2.