Tissue Inhibitor of Metalloprotease 1 Human Recombinant, HEK

Product Name: Tissue Inhibitor of Metalloprotease 1 Human Recombinant, HEK
Synonyms: Metalloproteinase inhibitor 1, Tissue inhibitor of metalloproteinases, TIMP-1, Erythroid-potentiating activity, EPA, TIMP1, CLGI, TIMP, EPO, HCI, FLJ90373.
Description: TIMP1 Human Recombinant produced in HEK-293 cells is a secreted protein with the sequence of Human TIMP-1 (amino acids Cys24-Ala207) and fused to a polyhistidine tag at the C-terminus.
Uniprot Accesion Number: P01033
Amino Acid Sequence:
Source: HEK293 Cells.
Physical Appearance and Stability: Lyophilized TIMP1 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution TIMP1 should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Formulation and Purity: The TIMP1 protein was lyophilized after extensive dialysis against PBS. Greater than 95.0% as determined by SDS-PAGE.
Application:
Solubility: It is recommended to reconstitute the lyophilized TIMP1 in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Biological Activity: The IC50 of 2.5-4 nM is measured by its ability to inhibit recombinant human MMP-2 cleavage of the colorimetric peptide substrate, Mca-PLGL-DpaAR-NH2.
Shipping Format and Condition: Lyophilized powder at room temperature.
Usage Statement: Our products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.