Hypoxanthine-Guanine Phosphoribosyltransferase, Human Recombinant, Active

Lyophilized powder at room temperature.

HPRT1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 238 amino acids (1-218 a.a) and having a molecular mass of 26.7kDa. HPRT1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MATRSPGVVI SDDEPGYDLD LFCIPNHYAE DLERVFIPHG LIMDRTERLA RDVMKEMGGH HIVALCVLKG GYKFFADLLD YIKALNRNSD RSIPMTVDFI RLKSYCNDQS TGDIKVIGGD DLSTLTGKNV LIVEDIIDTG KTMQTLLSLV RQYNPKMVKVASLLVKRTPR SVGYKPDFVG FEIPDKFVVG YALDYNEYFR DLNHVCVISE TGKAKYKA
Physical Appearance and Stability: Store at 4C if entire vial will be used within 2-4 weeks. Store, frozen at -20C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Formulation and Purity: HPRT1 protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0) and 20% glycerol. Greater than 95.0% as determined by SDS-PAGE.
Biological Activity: Specific activity is > 15 units/mg and is defined as the amount of enzyme that catalyze the formation of 1 umole of guanosine 5¿-monophosphate (GMP) per minute from guanine and phosphoribosyl pyrophosphate at pH 7.5 at 37C.