Macrophage Colony Stimulating Factor Human Recombinant, HEK

Lyophilized powder at room temperature.

M-CSF Human Recombinant produced in HEK cells is a glycosylated homodimer, having a molecular weight range of 35-40kDa due to glycosylation.The M-CSF is purified by proprietary chromatographic techniques.
Physical Appearance and Stability: Lyophilized M-CSF although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution M-CSF should be stored at 4C between 2-7 days and for future use below -18C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Formulation and Purity: The M-CSF was lyophilized from a 0.2um filtered solution containing 0.67mg/ml in 1xPBS. Greater than 95% as obsereved by SDS-PAGE.
Solubility: It is recommended to reconstitute the lyophilized M-CSF in sterile 1xPBS containing 0.1% endotoxin-free recombinant HSA.
Biological Activity: The specific activity was determined by the dose-dependent stimulation of the proliferation of murine M-NFS-60 cells (Mouse Myeloid Leukemia indicator cell line) and is 1.13ng/ml.