Description |
Product Name: Granulocyte Macrophage-Colony Stimulating Factor Human Recombinant Synonyms: CSF-2, MGI-1GM, GM-CSF, Pluripoietin-alpha, MGC131935, MGC138897. Description: Granulocyte Macrophage Colony Stimulating Factor Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 127 amino acids and having a molecular mass of 14477 Dalton. GM-CSF is purified by proprietary chromatographic techniques. Uniprot Accesion Number: P04141 Amino Acid Sequence:The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Ala-Arg-Ser.N-terminal methionine has been completely removed enzymatically. Source: Escherichia Coli. Physical Appearance and Stability: Lyophilized Granulocyte Macrophage Colony Stimulating Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution GMCSF should be stored at 4C between 2-7 days and for future use below -18C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. Formulation and Purity: GM-CSF was lyophilized after extensive dialysis against 2mM sodium phosphate buffer pH= 7.4+/-0.1. Greater than 98.0% as determined by:1. Analysis by RP-HPLC.2. Analysis by SDS-PAGE. Application: Solubility: It is recommended to reconstitute the lyophilized Granulocyte Macrophage Colony Stimulating Factor in sterile 18MO-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. Biological Activity: The ED50 as determined by the dose-dependant stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is < 0.1 ng/ml, corresponding to a Specific Activity of 11, 100, 000 IU/mg. Shipping Format and Condition: Lyophilized powder at room temperature. Usage Statement: Our products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |