| Description |
Product Name: Granulocyte Macrophage-Colony Stimulating Factor Rat Recombinant
Synonyms: CSF-2, MGI-1GM, GM-CSF, Pluripoietin-alpha, Molgramostin, Sargramostim.
Description: Granulocyte Macrophage Colony Stimulating Factor Rat Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 128 amino acids and having a molecular mass of 14590.65 Dalton. GM-CSF Rat Recombinant is purified by proprietary chromatographic techniques.
Uniprot Accesion Number: P48750
Amino Acid Sequence:The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Pro-Thr-Arg.
Source: Escherichia Coli.
Physical Appearance and Stability: Lyophilized Granulocyte Macrophage Colony Stimulating Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution GMCSF should be stored at 4C between 2-7 days and for future use below -18C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Formulation and Purity: GM-CSF Rat was lyophilized with no additives. Greater than 95.0% as determined by:(a)Analysis by RP-HPLC.(b)Analysis by SDS-PAGE.
Application:
Solubility: It is recommended to reconstitute the lyophilized Granulocyte Macrophage Colony Stimulating Factor in sterile 18MO-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Biological Activity: The ED50 range = 0.001-0.01ng/ml corresponding to a specific activity of 100, 000, 000-1, 000, 000, 000IU/mg, determined by the dose dependent proliferation of murine MC/9 cells.
Shipping Format and Condition: Lyophilized powder at room temperature.
Usage Statement: Our products are furnished forLABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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