Description |
Product Name: Granulocyte Macrophage-Colony Stimulating Factor Canine Recombinant Synonyms: CSF-2, MGI-1GM, GM-CSF, Pluripoietin-alpha, MGC131935, MGC138897. Description: GMCSF k9 Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 128 amino acids and having a molecular mass of 14.2 kDa. GM-CSF is purified by proprietary chromatographic techniques. Uniprot Accesion Number: P48749 Amino Acid Sequence:APTRSPTLVT RPSQHVDAIQ EALSLLNNSN DVTAVMNKAV KVVSEVFDPEGPTCLETRLQ LYKEGLQGSL TSLKNPLTMM ANHYKQHCPP TPESPCATQNINFKSFKENL KDFLFNIPFD CWKPVKK. Source: Escherichia Coli. Physical Appearance and Stability: Lyophilized GMCSF although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution GMCSF should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. Formulation and Purity: GMCSF was lyophilized after extensive dialysis against 1xPBS pH 7.4. Greater than 96.0% as determined by1. Analysis by RP-HPLC.2. Analysis by SDS-PAGE. Application: Solubility: It is recommended to reconstitute the lyophilized GMCSF in sterile 18M?-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. Biological Activity: The ED50 as calculated by the dose-dependent stimulation of the proliferation of human TF1 erythroleukemic cells is typically 1-4 ng/ml. Shipping Format and Condition: Lyophilized powder at room temperature. Usage Statement: Our products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |