Granulocyte Macrophage-Colony Stimulating Factor Human Recombinant, CHO

Lyophilized powder at room temperature.

GM CSF Human Recombinant produced in CHO cells is a 14.6kDa globular protein consisting of 127 amino acids, having two intramolecular disulfide bonds and two N-linked glycosylation sites.
Amino Acid Sequence:APARSPSPST QPWEHVNAIQ EARRLLNLSR DTAAEMNETV EVISEMFDLQ EPTCLQTRLE LYKQGLRGSL TKLKGPLTMM ASHYKQHCPP TPETSCATQI ITFESFKENL KDFLLVIPFD CWEPVQE
Source: Chinese Hamster Ovary cells.
Physical Appearance and Stability: Store at 4C if entire vial will be used within 2-4 weeks. Store, frozen at -20C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Formulation and Purity: The GM CSF Human filtered solution (0.27mg/ml) contains 20mM TRIS HCl buffer pH 7.2. Greater than 95% as determined by SDS-PAGE.
Biological Activity: ED50 < 0.1 ng/ml, determined by the dose dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).