Epidermal Growth Factor (Leu-21) Human Recombinant

Product Name: Epidermal Growth Factor (Leu-21) Human Recombinant
Synonyms: Urogastrone, URG, EGF.
Description: EGF 21-Leu Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 53 amino acids and having a molecular mass of 6205 Dalton.The EGF 21-Leu is purified by proprietary chromatographic techniques.
Uniprot Accesion Number:
Amino Acid Sequence:The sequence of the first five N-terminal amino acids was determined and was found to be Asn-Ser-Asp-Ser-Glu.
Source: Escherichia Coli.
Physical Appearance and Stability: Lyophilized Epidermal Growth Factor 21 Leu although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution EGF 21-Leu should be stored at 4C between 2-7 days and for future use below -18C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Formulation and Purity: The protein was lyophilized from a concentrated (1mg/ml) solution with no additives. Greater than 98.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Application:
Solubility: It is recommended to reconstitute the lyophilized Epidermal Growth Factor 21-Leu in sterile 18MO-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Biological Activity: The ED50, calculated by the dose-dependant proliferation of MDCK cells is < 10ng/ml concentration corresponding to a Specific Activity of 100, 000IU/mg.
Shipping Format and Condition: Lyophilized powder at room temperature.
Usage Statement: Our products are furnished for LABORATORY RESEARCH USE ONLY.They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.