Epidermal Growth Factor Rat Recombinant

Lyophilized powder at room temperature.

Epidermal Growth Factor Rat Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 53 amino acids and having a molecular mass of 6151 Dalton. The Rat EGF is purified by proprietary chromatographic techniques.
Amino Acid Sequence:NSNTGCPPSY DGYCLNGGVC MYVESVDRYV CNCVIGYIGE RCQHRDLRWW KLR.
Physical Appearance and Stability: Lyophilized Rat EGF although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution Rat EGF should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Formulation and Purity: Rat EGF was lyophilized from a 0.2um filtered concentrated (1.0mg/ml) solution in PBS, pH 7.4. Greater than 98.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Solubility: It is recommended to reconstitute the lyophilized Rat EGF in sterile water not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Biological Activity: The ED50 as calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells is less than 0.1ng/ml, corresponding to a specific activity of > 10, 000, 000 units/mg.