Fibroblast Growth Factor-18 Mouse Recombinant

Product Name: Fibroblast Growth Factor-18 Mouse Recombinant
Synonyms: Fibroblast growth factor 18, FGF-18, zFGF5, Fgf18, D130055P09Rik.
Description: FGF-18 Mouse Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 180 amino acids and having a molecular mass of 21kDa.The FGF-18 is purified by proprietary chromatographic techniques.
Uniprot Accesion Number: O89101
Amino Acid Sequence:EENVDFRIHV ENQTRARDDV SRKQLRLYQL YSRTSGKHIQ VLGRRISARG EDGDKYAQLL VETDTFGSQV RIKGKETEFY LCMNRKGKLV GKPDGTSKEC VFIEKVLENN YTALMSAKYS GWYVGFTKKG RPRKGPKTRE NQQDVHFMKR YPKGQAELQK PFKYTTVTKR SRRIRPTHPG.
Source: Escherichia Coli.
Physical Appearance and Stability: Lyophilized FGF-18 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution FGF-18 should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Formulation and Purity: FGF-18 protein was lyophilized from a 0.2um filtered concentrated solution in PBS, pH 7.4. Greater than 95.0% as determined by: (a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Application:
Solubility: It is recommended to reconstitute the lyophilized FGF-18 in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Biological Activity: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF-receptors is  < 0.5ng/ml, corresponding to a specific activity of  > 2.0x106 units/mg.
Shipping Format and Condition: Lyophilized powder at room temperature.
Usage Statement: Our products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.