Fibroblast Growth Factor-Acidic Rat Recombinant

Lyophilized powder at room temperature.

Fibroblast Growth Factor-acidic Rat Recombinant (FGF-1) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 141 amino acids and having a molecular mass of 15.9 kDa.The FGF acidic is purified by proprietary chromatographic techniques.
Amino Acid Sequence:MFNLPLGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SAGEVYIKGT ETGQYLAMDT EGLLYGSQTP NEECLFLERL EENHYNTYTS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D.
Physical Appearance and Stability: Lyophilized Fibroblast Growth Factor-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution FGF-a should be stored at 4C between 2-7 days and for future use below -18C.Please prevent freeze-thaw cycles.
Formulation and Purity: Lyophilized at a concentration of 1 mg/ml in 5mM Na2PO4, pH-7.5 and 50mM NaCl. Greater than 98.0% as determined by SDS-PAGE.
Solubility: It is recommended to reconstitute the lyophilized Fibroblast Growth Factor-acidic in sterile 18MO-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Biological Activity: The ED50 as determined by the dose-dependent proliferation of mouse BALB/c 3T3 cells, is less than 0.2ng/ml corresponding to a Specific Activity of 5x106IU/mg.